Associations of Widowhood and β-Amyloid With Cognitive Decline in Cognitively Unimpaired Older Adults

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Question  Is widowhood a specific risk factor associated with more rapid cognitive decline among cognitively unimpaired older adults with higher levels of brain β-amyloid, the Alzheimer disease biomarker?

Findings  In this cohort study of 257 community-dwelling cognitively unimpaired older adults, widowhood and β-amyloid were additively and interactively associated with cognitive decline. These results were independent of demographic factors, cardiovascular disease risk, depression, health-related behaviors, and social support factors.

Meaning  These findings suggest that widowhood may be an understudied risk factor for cognitive decline associated with Alzheimer disease and highlight the need for increased research and clinical attention to this high-risk group.

Importance  To reduce the rising incidence of clinical impairment due to Alzheimer disease, it is essential to define older adults at highest risk. Widowhood may be an unrecognized factor contributing to accelerated clinical progression along the Alzheimer disease pathway among cognitively unimpaired older adults.

Objective  To determine whether widowhood status and level of brain β-amyloid (ie, the Alzheimer disease pathologic protein) are additively or interactively associated with cognitive decline among cognitively unimpaired older adults.

Design, Setting, and Participants  In this cohort study, 257 married, widowed, and unmarried (ie, never married, divorced, or separated) participants from the Harvard Aging Brain Study longitudinal cohort underwent baseline evaluation of neocortical β-amyloid levels using Pittsburgh compound B positron emission tomography and 4 annual cognitive assessments. Data were collected from September 2010 to February 2017 and analyzed from July 2018 to July 2019.

Main Outcomes and Measures  Cognitive performance was measured using the Preclinical Alzheimer Cognitive Composite.

Results  Of the 257 participants, 153 (59.5%) were women, and the mean (SD) age was 73.5 (6.1) years; 145 participants (56.4%) were married (66 [45.5%] women), 77 (30.0%) were unmarried (56 [72.7%] women), and 35 (13.6%) were widowed (31 [88.6%] women). Compared with married participants, widowed participants demonstrated worsening cognitive performance after adjusting for age, sex, socioeconomic status, depression, and β-amyloid levels (β = −0.11; 95% CI, −0.19 to −0.04; P = .002) with no difference observed between married and unmarried participants. Furthermore, widowed participants with higher baseline β-amyloid levels exhibited steeper cognitive decline (β = −0.22; 95% CI, −0.42 to −0.03; P = .02), indicating both independent and interactive associations of β-amyloid levels and widowhood with cognition. In a secondary model using dichotomous β-amyloid–marital status groupings, the rate of cognitive decline among widowed participants with high β-amyloid was nearly 3 times faster than among married participants with high β-amyloid (widowed, high β-amyloid: β, −0.33; 95% CI, −0.46 to −0.19; P < .001; married, high β-amyloid: β, −0.12; 95% CI, −0.18 to −0.01; P < .001).

Conclusions and Relevance  In a sample of cognitively unimpaired older adults, being widowed was associated with accelerated β-amyloid–related cognitive decline during 3 years. Cognitively unimpaired, widowed older adults were particularly susceptible to Alzheimer disease clinical progression, emphasizing the need for increased research attention and evidenced-based interventions for this high-risk group.

Alzheimer disease (AD) dementia is an urgent and global public health challenge, a condition that affects 50 million men and women worldwide and is projected to triple in prevalence by 2050.¹ To reduce the incidence and consequences of AD dementia, it is essential to recognize and develop preventive treatments for older adults at high risk of AD-related cognitive decline.

Widowed older men and women are a demographic group susceptible to cognitive decline. They represent more than 11% of men and 34% of women aged 65 years or older living in the United States.² Widowhood has been independently associated with declines in memory performance^3-6 and an increased risk of incident dementia.⁷ Using prospective data from the US Health and Retirement Study, Shin et al⁵ evaluated more than 6000 married older adults and observed significantly greater cognitive decline among those who became widowed compared with those who did not, adjusting for age, race/ethnicity, sex, education, depression, other health conditions and behaviors, spouse’s medical status before death, and remarriage. Differences in cognitive performance were significant 2 years after spousal loss and worsened with longer duration of widowhood.⁵ A meta-analysis of 15 studies including 812 047 participants⁷ found that widowed men and women had a 20% greater risk of developing dementia compared with married persons during 3 to 15 years of follow-up.

Despite strong evidence linking late-life widowhood with cognitive decline and dementia, the underlying mechanisms are not well understood. Moreover, to our knowledge, the association of widowhood with cognition in individuals with biomarker evidence of early AD pathology has not been investigated.

Alzheimer disease is biologically defined by the presence of brain deposits of β-amyloid and tau pathologies, which initially accumulate while individuals have no cognitive impairment.^8,9 Approximately 25% of cognitively unimpaired adults aged 60 years or older have elevated levels of β-amyloid, as detected by positron emission tomography (PET), and are 2 to 5 times more likely to progress to clinical impairment (ie, mild cognitive impairment or dementia) than those with low β-amyloid levels during 3 to 4 years of follow-up.^10,11 Increased but variable rates of progression to clinical impairment among individuals with high β-amyloid levels suggest the importance of identifying other biological and clinical factors that might explain the heterogeneity of cognitive outcomes. At the same time, understanding the contribution of β-amyloid to cognitive outcomes in specific at-risk populations, such as widowed older adults, is also clinically important.

In this study, we evaluated short-term changes in cognitive performance among cognitively unimpaired, widowed older adults compared with their married counterparts and whether rates of cognitive change were further influenced by β-amyloid levels. We hypothesized an interactive association of widowhood and β-amyloid with cognition in which widowhood would be associated with worsening cognition compared with the married group, independent of age, sex, socioeconomic status, depression, and baseline β-amyloid levels, and rates of cognitive change would be accelerated among those with higher levels of β-amyloid. In secondary analyses, we tested whether the association of widowhood and cognitive change was independent of several biological, behavioral, and social support factors.

The sample included 257 community-dwelling cognitively unimpaired men and women participating in the Harvard Aging Brain Study, an observational, multimodal imaging study of cognition in aging and early AD. Screening procedures and inclusion and exclusion criteria for the cohort have previously been described¹² (eAppendix in the Supplement). All participants were cognitively unimpaired at enrollment, based on a Clinical Dementia Rating¹³ global score of 0 and normal education-adjusted neuropsychological test performance.^14,15 All participants scored below the cutoff for mild depression on the Geriatric Depression Scale (GDS), 30-item version.¹⁶ We included all Harvard Aging Brain Study participants who had completed 4 annual assessments in this study (eAppendix in the Supplement).

The Partners Human Research Committee approved this study. All participants provided written informed consent prior to enrollment. This report follows the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) reporting guideline.

All participants reported their current marital status at the baseline study visit by selecting from the following marital categories: single, married, divorced, separated, widowed, never married, unknown, or other (eAppendix in the Supplement). For the main analyses, participants were classified into 1 of the 3 following groups: married, unmarried (ie, divorced, separated, single, or never married), or widowed. To address possible differences in cognitive outcomes within the unmarried group and to further test the specificity of the association of widowhood with cognitive change, we classified participants into the 4 following groups in supplementary models: married, divorced or separated, single or never married, and widowed.

All participants were evaluated at 4 annual study visits using the Preclinical Alzheimer Cognitive Composite (PACC).¹⁷ The PACC score was computed as the mean z score from the Logical Memory Delayed Recall, a test of story recall, presented orally (0-25 story units)¹⁴; the modified Mini-Mental State Examination, a measure of global cognition (0-30 points)¹⁵; the Wechsler Adult Intelligence Scale–Revised Digit Symbol coding, a test of timed executive function scored during 90 seconds (0-93 symbols)¹⁸; and the Free and Cued Selective Reminding Test, an associative memory task measuring both free recall and recall with semantic cues, scored as the sum of the free recall and total recall components (0-96 words)^17,19 (eAppendix in the Supplement). A higher PACC z score denoted better cognitive function. The PACC has been found to be sensitive to incremental declines in cognition among cognitively unimpaired older adults with elevated β-amyloid levels.^11,17 As the Logical Memory Delayed Recall story is repeated yearly, improved PACC performance attributable to practice effects has previously been reported in Harvard Aging Brain Study participants with low β-amyloid levels.^17,20

Socioeconomic status was assessed using the Two-Factor Hollingshead score, on which a higher score indicates lower socioeconomic status.²¹ Depression was measured at baseline and annually using the 30-item GDS. History of any episode of depressive disorder was scored as absent or present. Cardiovascular disease risk score was derived from a weighted sum of age, sex, antihypertensive treatment, systolic blood pressure, body mass index (calculated as weight in kilograms divided by height in meters squared), history of diabetes, and current cigarette smoking status.^22,23

Level of social engagement was assessed at baseline using 5 questions from the Community Healthy Activities Model Program for Seniors questionnaire, which measures time spent per week with family, friends, and in community activities (range, 0-30).^12,24 Analogous scoring for 27 items on the Community Healthy Activities Model Program for Seniors pertaining to sports, exercise, and dance were combined to calculate a baseline physical activity score (range, 0-162).

Level of emotional support from children, relatives, and friends was assessed by 3 questionnaire items,²⁵ which asked participants to specify numbers of close relationships with children, relatives, and friends during the fourth annual assessment (eAppendix in the Supplement). As these data were not collected at earlier assessments, these responses were used as proxy baseline data for the purpose of this study.

All magnetic resonance imaging was performed at the Massachusetts General Hospital, Athinoula A. Martinos Center for Biomedical Imaging on a 3-T imaging system (TIM Trio; Siemens) with a 12-channel phased-array head coil. The protocol consisted of a T1-weighted volumetric magnetization–prepared rapid-acquisition gradient-echo sequence (repetition time, 2300 milliseconds; echo time, 2.95 milliseconds; inversion time, 900 milliseconds; flip angle, 9°; 1.05 × 1.05 × 1.2 mm resolution). We processed and quality-assessed T1-weighted images using FreeSurfer version 6.0.0 with the software package’s default automated reconstruction protocol, as described previously.^26-28 Bilateral hippocampal volumes were extracted and adjusted for the estimated intracranial volume (eTIV) using the following equation²⁹: adjusted hippocampal volume = raw hippocampal volume − b(eTIV − mean eTIV), in which b indicates the regression coefficient when hippocampal volume is regressed against eTIV.

We measured β-amyloid burden using Pittsburgh compound B (PiB)-PET protocols, as previously described.³⁰ Briefly, PET data were reconstructed and attenuation-corrected using standard Siemens software. We calculated PiB retention³¹ using a gray matter cerebellum reference region and applied a geometric transfer matrix partial volume correction.³² Neocortical β-amyloid deposition was quantified using an aggregate partial volume correction distribution volume ratio from a set of FreeSurfer regions including frontal, lateral parietal, lateral temporal, and retrosplenial cortices.³⁰ Dichotomous high β-amyloid and low β-amyloid groups were defined by Gaussian mixture modeling, resulting in a threshold value of greater than 1.32 PiB distribution volume ratio for the high β-amyloid group.³³ A continuous measure of β-amyloid was used in the main analyses.

All statistical analyses were performed using R software version 3.6.1 (R Project for Statistical Computing). Baseline values for demographic, clinical, and imaging variables were compared across marital groups using analysis of variance, Kruskal-Wallis, Tukey, χ², and Fisher exact tests.

In the first main linear mixed-effects model, we examined the association of marital status with longitudinal PACC scores, including marital status, time, and its interaction as fixed effects and individual intercepts and slopes for time in years as random effects. The unmarried and widowed groups were compared with the married reference group to estimate differences in longitudinal PACC scores using the maximum likelihood estimation, controlling for age, sex, socioeconomic status, depression history, depressive symptoms, β-amyloid level, and their interactions with time. A second main model examined the association of the 3-way multiplicative interaction of marital status, continuous β-amyloid level, and time with longitudinal PACC scores, controlling for the same covariate-time interactions and main terms.

Interactions of β-amyloid levels and marital status were further evaluated in a secondary model using 6 marital status and dichotomous β-amyloid groups to estimate longitudinal PACC scores, controlling for the same covariates. The 6 groups were married, low β-amyloid (99 participants; reference group); married, high β-amyloid (44 participants); unmarried, low β-amyloid (53 participants); unmarried, high β-amyloid (23 participants); widowed, low β-amyloid (28 participants); and widowed, high β-amyloid (7 participants).

Secondary analyses probed the specificity of the association of marital status with cognitive and neurobehavioral outcomes. These analyses, analogous to the main models, estimated longitudinal scores for each of the PACC component tests (rather than the composite PACC score) or for longitudinal GDS scores as separate dependent variables, with marital status and its interaction with time or its interaction with time and β-amyloid level as the variables of interest. Additional secondary models adjusted for biological factors (ie, hippocampal volume and cardiovascular risk score), behavioral factors (ie, level of physical exercise and social engagement), or social support (ie, numbers of close relationships) to test whether these covariates contributed to the association of widowhood with cognition.

For linear mixed-effects models, we report unstandardized coefficient estimates (β) with 95% CIs, t statistics or effect sizes (ie, Cohen d), and P values. We considered P < .05 statistically significant, and all tests were 2-tailed. Residual-vs-fits and normality plots were evaluated for each variable and model to ensure that distributions reasonably satisfied model assumptions of normality and homoscedasticity.

Of 257 participants, 153 (59.5%) were women, and the mean (SD) age was 73.5 (6.1) years; 145 participants (56.4%) were married (66 [45.5%] women), 77 (30.0%) were unmarried (56 [72.7%] women), and 35 (13.6%) were widowed (31 [88.6%] women). Baseline data and statistical comparisons across marital status groups are shown in Table 1. Compared with the married group, the widowed group was older (mean [SD] age, 73.3 [5.8] years vs 77.6 [6.6] years; P < .001), had proportionally more women (66 [45.5%] vs 56 [72.7%]; P < .001), and had lower socioeconomic status (mean [SD] Hollingshead score, 24.4 [14.0] vs 31.4 [13.7]; P = .03). The widowed group was also older than the unmarried group (mean [SD] age, 77.6 [6.6] years vs 72.1 [5.5]; P < .001) but did not differ from the unmarried group by sex or socioeconomic status. There were no baseline group differences in mean PiB, GDS score, PACC z scores, or depression history. Compared with the married group, the widowed group had higher social engagement scores (mean [SD], 7.5 [4.0] vs 9.5 [3.9]; P = .04), but the groups did not differ in physical activity or close relationship scores (eTable 1 in the Supplement). There were no significant group differences in lifetime prevalence of hypertension, diabetes, cardiovascular or cerebrovascular conditions, hearing loss, alcohol or substance use disorders, or current smoking or alcohol use (eTable 2 in the Supplement).

During the 3 years of observation, mean (SD) change in unadjusted PACC z score was 0.13 (0.56) for the married group, 0.07 (0.48) for the unmarried group and −0.16 (0.72) for the widowed group (unmarried compared with married: t₁₇₇ = 0.90; P = .37; Cohen d = 0.12; widowed compared with married: t₄₄ = 2.25; P = .03; Cohen d = 0.50; widowed compared with unmarried: t₄₈ = −1.71; P = .09; Cohen d = 0.40) (Figure 1).

In the first mixed-effects model, cognitive performance on PACC declined in the widowed group, differing significantly from the married group (β, −0.11; 95% CI, −0.19 to −0.04; P = .002) (Table 2 and Figure 2A). Longitudinal PACC scores did not differ between the married and unmarried groups (β, −0.03; 95% CI, −0.08 to 0.02; P = .21) (Table 2 and Figure 2A). Higher β-amyloid level (β, −0.14; 95% CI, −0.19 to −0.08; P < .001) and older age (β, −0.004; 95% CI, −0.01 to −0.00004; P = .045) were also associated with declining PACC scores in this model (Table 2).

In the second main model, β-amyloid–associated PACC decline was steeper in the widowed group compared with the married group (β, −0.22; 95% CI, −0.42 to −0.03; P = .02) but did not differ between the unmarried and married groups (β, −0.01; 95% CI, −0.14 to 0.12; P = .91) (Table 2). Results indicated a specific and synergistic association of the widowed group and higher β-amyloid level with PACC decline beyond their independent associations.

Results were consistent in models using β-amyloid as a dichotomous grouping variable. Longitudinal PACC scores worsened in the widowed, low–β-amyloid group compared with the married, low–β-amyloid reference group (β, −0.09; 95% CI, −0.17 to −0.009; P = .03) but did not differ between the unmarried, low–β-amyloid and married, low–β-amyloid groups (β, −0.05; 95% CI, −0.11 to 0.01; P = .12) (Figure 2B; eTable 3 in the Supplement). Moreover, longitudinal PACC scores were worse in all 3 high–β-amyloid groups compared with the married, low–β-amyloid reference group (married, high β-amyloid: β, −0.12; 95% CI, −0.18 to −0.06; P < .001; unmarried, high β-amyloid: β, −0.10; 95% CI, −0.18 to −0.02; P = .01; widowed, high β-amyloid: β, −0.33; 95% CI, −0.46 to −0.19; P < .001) (Figure 2B; eTable 3 in the Supplement). These β estimates indicate that the rate of PACC decline in the widowed, high–β-amyloid group was nearly 3-fold faster than in the married, high β-amyloid group.

Results for the main models remained significant in sensitivity analyses removing the widowed participant with lowest cognitive function and trajectory or removing all participants who changed marital status (data not shown). Models using 4 rather than 3 marital categories also showed consistent results for the widowed and unmarried subgroups (eTable 4, eTable 5, and eFigure in the Supplement).

Widowhood, but not depression, was associated with declining cognition in the main analyses (GDS × time: β, 0.004; 95% CI, −0.004 to 0.01; P = .29; depression history × time: β, 0.02; 95% CI −0.05 to 0.08; P = .65). In a secondary analysis examining the association of marital status with depression as the outcome, no association was found between marital status and depression (ie, GDS) scores over time (eTable 6 in the Supplement).

Examining marital status with each PACC component test as separate outcomes, widowhood was significantly associated with worsening performance on the Logical Memory Test (β, −0.53; 95% CI, −0.96 to −0.09; P = .02) and the Free and Cued Selective Reminding Test (β, −0.27; 95% CI, −0.48 to −0.07; P = .01). Associations did not reach significance with other tests (eTable 7 in the Supplement).

The association of widowhood with declining PACC scores remained significant in models that included β-amyloid level as well as cardiovascular disease risk score, hippocampal volume, and their interactions with time. Results of this model indicated independent associations of widowhood (β, −0.12; 95% CI, −0.19 to −0.04; P = .005), higher cardiovascular disease risk score (β, −0.003; 95% CI, −0.005 to −0.0005; P = .02), and greater β-amyloid level (β, −0.13; 95% CI, −0.19 to −0.07; P < .001) but not hippocampal volume (β, <0.001; 95% CI, −0.00003 to 0.00004; P = .94) with worsening PACC scores (Table 3).

The association of widowhood with PACC scores remained significant when covarying for physical activity scores, social engagement scores, and their interaction with time (β, −0.13; 95% CI, −0.19 to −0.06; P < .001), and these additional terms were not associated with longitudinal PACC scores (physical activity: β, 0.001; 95% CI, −0.0004 to 0.003; P = .14; social engagement: β, −0.0003; 95% CI, −0.005 to 0.005; P = .90) (Table 3). The widowhood × time association with PACC scores also remained significant in a model covarying for number of close relationships and its interaction with time (β, −0.10; 95% CI, −0.17 to −0.04; P = .002). The close relationships × time interaction was not associated with PACC scores (β, 0.004; 95% CI, −0.0002 to 0.008; P = .07) (Table 3).

The purpose of this study was to evaluate the associations of widowhood, β-amyloid levels, and cognitive function in a sample of cognitively unimpaired older adults. High β-amyloid levels were associated with cognitive decline in all marital groups during 3 years of observation, with a much steeper rate of decline among participants who were widowed. The widowed, high–β-amyloid group declined 3 times faster than the married, high–β-amyloid group, independent of age, sex, socioeconomic status, and depression. Cognitive trajectories also differed between married and widowed participants with low β-amyloid, pointing to both independent and synergistic associations of widowhood and β-amyloid levels with cognition over time. There were no differences in cognitive outcomes between married and unmarried (ie, nonwidowed) participants. These findings illustrate the importance of widowhood as a clinically relevant risk factor for cognitive decline, and they provide new evidence that widowed men and women are a distinct subgroup of older adults who are particularly susceptible to progression in early AD.

Both risk of widowhood and risk of AD dementia increase with advancing age, particularly among women. Recent US census data have documented that widows include 6.4% of men and 19.5% of women aged 65 to 74 years, 14.7% of men and 42.9% of women aged 75 to 84 years, and 35.3% of men and 71.9% of women aged 85 years or older.² Thus, widowhood may contribute to the excess AD dementia risk observed in older women, but this association is largely unrecognized. Importantly, these data support that both male and female widowed older adults are a large and readily identified demographic group who may be at high risk of AD-related cognitive decline and merit both research and clinical attention.

Death of a spouse is considered to be among the most stressful life events.³⁴ Early sequelae include painful feelings of loss,³⁵ sadness,³⁶ and sleep disturbance, particularly in the context of greater depressive symptoms.^37,38 Physiologic arousal, manifested as higher heart rate,³⁹ higher systolic blood pressure,³⁹ and elevation in morning blood cortisol level,⁴⁰ can also occur and persist for months after spousal loss.⁴¹ Other reported changes include increased platelet activation,⁴² higher levels of proinflammatory cytokines,⁴³ and alterations in cellular immune response.^40,44 It is well established that risk of myocardial infarction, stroke, nonacute coronary syndrome, and pulmonary embolism are elevated in the days and months following spousal loss.⁴⁵ These early behavioral, cardiovascular, and inflammatory alterations may plausibly interact or act in parallel with neurodegenerative processes, such as AD, to lower the threshold for cognitive decline; however, relatively little is known of behavioral or physiologic sequelae beyond this early period and how such changes might specifically relate to ongoing cognitive decline.⁴⁶

We found no evidence that the association of widowhood with cognitive decline was mediated by depression history or depressive symptom burden in this sample. This is consistent with other observational studies, which have reported independent associations of widowhood and depression with cognitive decline^3,6 and risk of cognitive impairment.⁴⁷

In this select, relatively healthy sample, widowed participants did not differ from other marital groups by health conditions or behaviors, age-adjusted hippocampal volumes, level of β-amyloid, or cardiovascular disease risk scores at baseline. Despite these baseline similarities, we found evidence that widowhood, level of β-amyloid, and cardiovascular disease risk score had independent and cumulative associations with longitudinal cognition at the individual level. Widowhood had a unique association with cognitive decline that was not accounted for by these other known biological risk factors.

Death of a spouse can pose a critical loss of intimacy, meaningfulness, companionship, and everyday support. Close relationships have the potential to buffer against the physiological and psychological effects of stress and provide opportunities for cognitive stimulation.²⁵ Close family relationships (eg, having a child or living sibling) have previously been found to affect cognitive outcomes in widowed people.^5,48

We found no association for number of close relationships, social engagement, or physical activity with longitudinal cognition. Negative findings in these secondary analyses should be interpreted cautiously because of the limits of sample size, duration of follow-up, and the overall cognitive stability of the sample.

This study has limitations. Widowed participants in the study were healthier than in the general population, which may have led to an underestimation of the association of widowhood with cognitive decline. We were unable to investigate sex-related differences within the widowed group, given that nearly all participants were women. These analyses did not take into account nonmarried partners, information on earlier marriages, marriage quality, cause of spousal death, or caregiver status. Furthermore, our study lacked inflammatory biomarker, sleep, or other physiologic data to elucidate possible mediators or moderators of the association of widowhood with cognitive decline. Lastly, as β-amyloid neuroimaging is primarily used for research purposes at this time, our findings do not have immediate relevance to the clinical care of older individuals. However, these findings may inform the design of new detection and prevention initiatives for unimpaired older adults using scalable, biomarker-based diagnostic tests for AD.

In this cohort study, we examined a cognitively unimpaired sample and observed declining cognitive function among widowed older adults compared with their married peers during 3 years, with more rapid declines in participants with higher levels of brain β-amyloid. Cardiovascular disease risk profile was further associated with cognitive trajectories among widowed participants. Widowhood is an underrecognized risk factor associated with AD-related cognitive decline and impairment. Further research and intervention strategies are needed to ameliorate psychosocial and biological processes underlying cognitive decline in widowed older adults.

Accepted for Publication: January 6, 2020.

Published: February 26, 2020. doi:10.1001/jamanetworkopen.2020.0121

Open Access: This is an open access article distributed under the terms of the CC-BY License. © 2020 Biddle KD et al. JAMA Network Open.

Corresponding Author: Nancy J. Donovan, MD, Division of Geriatric Psychiatry, Department of Psychiatry, Brigham and Women’s Hospital, 60 Fenwood Rd, 4120, Boston, MA 02115 (njdonovan@bwh.harvard.edu).

Author Contributions: Ms Biddle and Dr Donovan had full access to all of the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis.

Concept and design: Biddle, Zide, Johnson, Donovan.

Acquisition, analysis, or interpretation of data: All authors.

Drafting of the manuscript: Biddle, d’Oleire Uquillas, Properzi, Johnson, Donovan.

Critical revision of the manuscript for important intellectual content: Biddle, Jacobs, d’Oleire Uquillas, Zide, Kirn, Rentz, Johnson, Sperling, Donovan.

Statistical analysis: Biddle, Jacobs, d’Oleire Uquillas, Zide, Johnson, Donovan.

Obtained funding: Johnson, Sperling, Donovan.

Administrative, technical, or material support: Properzi, Johnson, Donovan.

Supervision: Jacobs, Kirn, Rentz, Johnson, Sperling, Donovan.

Conflict of Interest Disclosures: Ms Biddle reported receiving speaking fees from Jazz Pharmaceuticals outside the submitted work. Dr Jacobs reported receiving grants from the European Union Horizon 2020 Research and Innovation Programme outside the submitted work. Dr Sperling reported receiving personal fees from AC Immune, Biogen, Neurocentria, Eisai, Roche Holding, Takeda Pharmaceutical Company, and Novartis outside the submitted work; receiving grants and personal fees from Janssen Pharmaceuticals outside the submitted work; and receiving grants from Eli Lilly and Co outside the submitted work. Dr Donovan reported receiving research salary support from Eli Lilly and Co and Eisai and serving on the advisory board for Avanir Pharmaceuticals outside the submitted work. No other disclosures were reported.

Funding/Support: This study was supported by grant R21 AG054953 from the National Institute on Aging to Dr Donovan and grant NIA P01 AG036694 from the Harvard Aging Brain Study to Drs Sperling and Johnson. Dr Jacobs receives funding from the European Union Horizon 2020 Research and Innovation Programme under the Marie Sklodowska-Curie Grant agreement (award number, IF-2015-GF, 706714).

Role of the Funder/Sponsor: The funders had no role in the design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, or approval of the manuscript; and decision to submit the manuscript for publication.

Meeting Presentation: This article was presented in part as an abstract at the 22nd Conference of the Alzheimer’s Association International Conference; July 16, 2019; Los Angeles, California.