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October 1960

Interferometric Study of Astrocytes in Gliosis and Astrocytoma

Author Affiliations

Jacksonville, Fla.
From the Research Laboratory, Baptist Memorial Hospital.

Arch Neurol. 1960;3(4):416-419. doi:10.1001/archneur.1960.00450040066009

Recent developments in light microscopy have permitted a new approach to the morphology of the nervous system by use of unstained tissue sections. Von Hirsch and Hager1,2 investigated aspects of the normal and pathological histology of the central nervous system with the use of phase-contrast microscopy. The same method was used by Calvo3 to study smears of brain tumors. Phase-contrast microscopy was demonstrated to be a useful tool for morphological, qualitative studies of histology.4 The later development of interference microscopy brought forth the possibility of adding quantitative determinations to the qualitative examination. Interferometric determination of cell thickness, dry cellular mass, and the results of the extraction of various substances from nuclei have been reported.5-9 Thorough discussions of the principles of interference microscopy, types of microscopes, and sources of error were made by Barer10 and by Hale.11 In our laboratory we have applied microscopic interferometry