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December 1960

Determination of Phospholipids in Spinal Fluid and Brain

Author Affiliations

Washington, D.C.
Department of Biological Chemistry, Georgetown University Schools of Medicine and Dentistry.

Arch Neurol. 1960;3(6):677-682. doi:10.1001/archneur.1960.00450060065007

Introduction  Cholesterol and phospholipids, constituents of myelin, may appear in spinal fluid as a result of demyelination. A micromethod for the determination of cholesterol in spinal fluid and its application in the study of multiple sclerosis have been reported.1,2 The concentration of phospholipids in spinal fluid is very low, and their determination has been hampered by the lack of adequate micromethods. Tourtellotte, in a comprehensive review,3 described some attempts of earlier investigators to determine the phospholipids in spinal fluid. The same investigator reported a quantitative study of lipids in spinal fluid by chemical procedures.4 The successful application of chromatography to the analysis of blood lipids suggested that it would be useful in the analysis of spinal fluid and brain. Using a silicic acid column, cephalin, lecithin, sphingomyelin and lysolecithin have been separated from both tissues and determined. Estimation of the first 3 phospholipid components in spinal fluid

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