In the metallic impregnation techniques, special fixation and very fresh material are often required, thus limiting their usefulness for routine formalin-fixed material obtained at necropsy. Most of the traditional silver impregnation methods for neurofibrils of ganglion cells and their processes, axons, and dendrites, require frozen-sectioning. Paraffin-embedded material can also be used. However, silver impregnation methods for nerve fibers in celloidin sections have not been used generally either because of the technical complexity involved, the wholly inadequate impregnations, or the inconstant staining results. In 1922, Brandt and Kadanoff1 applied Schultz's sodium hydroxide-silver method to celloidin, paraffin, and gelatin-embedded sections and examined the effects of varying the time of impregnation and the concentration of the silver solution. The application of silver to human nerve fiber impregnation in celloidin sections, however, was not made until 1929 when it was first reported by Davenport.2 He succeeded in staining nerve fibers in celloidin-embedded
HIRANO A, ZIMMERMAN HM. Silver Impregnation of Nerve Cells and Fibers in Celloidin Sections: A Simple Impregnation Technique. Arch Neurol. 1962;6(2):114–122. doi:10.1001/archneur.1962.00450200028003
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