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Article
March 1962

Nucleic Acid and Protein Metabolism in White Matter: Observations During Experimental Demyelination and Remyelination; a Histochemical and Autoradiographic Study of Spinal Cord of the Adult Cat

Author Affiliations

CHICAGO; NEW YORK
From the Neurology and the Radioisotope Services, Veterans Administration Research Hospital, and the Department of Neurology and Psychiatry, Northwestern University Medical School, Chicago (Dr. Koenig), and the Department of Anatomy, the Medical School, and the Department of Zoology, University of Wisconsin, Madison (Dr. R. P. Bunge and Dr. M. B. Bunge).
Postdoctoral Fellow of the National Institute of Neurological Diseases and Blindness (Dr. M. B. Bunge); Postdoctoral Fellow of the National Multiple Sclerosis Society (Dr. R. P. Bunge). The Doctors Bunge are presently in the Department of Anatomy and the Laboratory for Cell Physiology, Columbia University College of Physicians and Surgeons.

Arch Neurol. 1962;6(3):177-193. doi:10.1001/archneur.1962.00450210005002
Abstract

Introduction  The experimental lesion produced by cerebrospinal fluid exchange provides an unusual opportunity to investigate demyelination and remyelination in the adult mammalian neuraxis. The lesion was discovered empirically when animals subjected to repeated withdrawal and reinjection of cerebrospinal fluid (CSF) were observed to develop conspicuous neurological deficits.1 Though the mechanism by which this lesion is produced remains unknown, the unique occurrence of remyelination following demyelination has provided impetus for light and electron microscopic studies1-3 and for this autoradiographic analysis. Taken together, these studies indicate that adult glial cells have a degree of versatility not hitherto recognized.To produce the lesion nothing is added to the CSF; it is simply withdrawn and reinjected repeatedly through a needle in the cisterna magna. A portion of animals so treated (∼40%) develop partial paralysis of the posterior extremities; the more severely affected are unable to walk. At autopsy the lesion is

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