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September 1962

Oxidative Histochemistry of Reactive Astrocytes

Author Affiliations

From the Department of Pathology, University of Minnesota Medical School.

Arch Neurol. 1962;7(3):211-218. doi:10.1001/archneur.1962.04210030049007

In the present report the results of a morphologic histochemical study of oxidative enzymes in reactive astrocytes will be presented. This investigation was undertaken as an effort to determine whether reactive astrocytes show a different pattern of enzymatic activities from normal cells and if so, the duration of such changes. For this purpose morphologic histochemical procedures were chosen since brain tissue is composed of many different cell types and major biochemical changes occurring in one cell type can be easily overlooked by classical methods of gross analysis.

Recent advances in tetrazolium salt techniques have made it possible to study a number of oxidative enzymes in histologic sections. Many colorless, or near colorless, water-soluble tetrazolium salts can serve as hydrogen acceptors in a number of oxidative enzymatic reactions, yielding highly colored insoluble formazan precipitates at the site of enzymatic activity.1-5 In the present work techniques employing tetrazolium salt reduction have