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December 1964

Fine Structure of Cerebral Fluid Accumulation: V. Transfer of Fluid From Extracellular to Intracellular Compartments in Acute Phase of Cryptococcal Polysaccharide Lesions

Author Affiliations

From the Henry and Lucy Moses Research Laboratories of the Laboratory Division, Montefiore Hospital, New York, and New York Medical College Center for Chronic Disease, New York.

Arch Neurol. 1964;11(6):632-641. doi:10.1001/archneur.1964.00460240064009

Introduction  Implantation of pellets of cryptococcal or pneumococcal capsular polysaccharides mixed with graphite into the rat forebrain resulted in selective morphological alterations in white matter.1 These lesions were due to spread of the implanted polysaccharide along the callosal radiation, as revealed by immunofluorescence.2 Electron microscopic observations of the involved white matter distant from the implant proved that the polysaccharides were located in the extracellular space during the acute stage.3 However, small amounts of polysaccharide invaded the intracellular compartment even during the initial phase of reaction, especially at the margins of the implant. The present study is concerned with the methods by which polysaccharide obtains access to the interior of cerebral cells. It will be demonstrated that there are two mechanisms for extracellular-intracellular transfer in the acute phase of the experiment.

Material and Methods  Pellets of cryptococcal polysaccharide mixed with equal parts of graphite were implanted into the