NTRACEREBRAL implants of cryptococcal or pneumococcal polysaccharides caused intense vacuolation in white matter.1 Light microscopic and immunofluorescent studies indicated that the polysaccharides spread in white matter for considerable distances.1,2 In the late stage, the vacuoles were clearly intracellular, and the same location was erroneously inferred for the early stage.1 Electronmicroscopic studies of the early stage proved that the vacuoles actually constituted greatly distended extracellular spaces filled with polysaccharide-rich fluid.3 Only small amounts of fluid entered cells, either by direct invasion through ruptured cell walls (only in the neighborhood of the implant), or by engulfment in relatively rare phagocytes.4 The present work is an electron microscopic study of the subacute and chronic phases of the experiment, phases in which the bulk of the fluid and foreign polysaccharide leave the extracellular spaces to become intracellular. In this work, we focus attention on the oligodendrocyte.
ASAO HIRANO, H. M. ZIMMERMAN, SEYMOUR LEVINE. Fine Structure of Cerebral Fluid AccumulationVI. Intracellular Accumulation of Fluid and Cryptococcal Polysaccharide in Oligodendroglia. Arch Neurol. 1965;12(2):189–196. doi:10.1001/archneur.1965.00460260079009