TISSUE culture provides an opportunity to study living cells under defined conditions. Survival following removal from their normal environment requires that cells adapt to the conditions imposed by tissue culture. In the process of adaptation, metabolic and morphologic changes occur abruptly and continue over the course of time. With awareness of the foregoing limitations, tissue culture has been employed widely as a means of studying living cells.
Tumors of the central nervous systems grow readily in tissue culture.1,2 Beginning in October 1962, we attempted to culture all intracranial and intraspinal tumors removed by operation. Monolayer cultures were selected rather than plasma clot cultures because the method is less complicated and duplicate cultures can be prepared in unlimited numbers. The purpose of this paper is to report our experience with monolayer tissue cultures of 125 neural tumors.
Tumor fragments are implanted within two hours following operative removal, although
WILSON CB, BARKER M, SLAGEL DE. Tumors of the Central Nervous System in Monolayer Tissue. Arch Neurol. 1966;15(3):275–282. doi:10.1001/archneur.1966.00470150053009
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