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September 1966

Tumors of the Central Nervous System in Monolayer Tissue

Author Affiliations

From the Department of Surgery, Division of Neurosurgery, University of Kentucky College of Medicine, Lexington.

Arch Neurol. 1966;15(3):275-282. doi:10.1001/archneur.1966.00470150053009

TISSUE culture provides an opportunity to study living cells under defined conditions. Survival following removal from their normal environment requires that cells adapt to the conditions imposed by tissue culture. In the process of adaptation, metabolic and morphologic changes occur abruptly and continue over the course of time. With awareness of the foregoing limitations, tissue culture has been employed widely as a means of studying living cells.

Tumors of the central nervous systems grow readily in tissue culture.1,2 Beginning in October 1962, we attempted to culture all intracranial and intraspinal tumors removed by operation. Monolayer cultures were selected rather than plasma clot cultures because the method is less complicated and duplicate cultures can be prepared in unlimited numbers. The purpose of this paper is to report our experience with monolayer tissue cultures of 125 neural tumors.

Methods  Tumor fragments are implanted within two hours following operative removal, although

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