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Article
February 1969

CSF Immunoglobulins in Multiple Sclerosis and Other Neurologic Diseases: Measurement by Electroimmunodiffusion

Author Affiliations

Denver
From the Division of Neurology (Dr. Schneck) and the Division of Clinical Immunology (Dr. Claman), University of Colorado Medical Center, Denver.

Arch Neurol. 1969;20(2):132-139. doi:10.1001/archneur.1969.00480080032003
Abstract

MORE than 25 years have passed since Kabat and co-workers,1 using the Tiselius electrophoresis method, first noted that the cerebrospinal fluid (CSF) γ-globulin content was elevated in some patients with multiple sclerosis and neurosyphilis. Many methods have been described subsequently in the search for a rapid, simple, and reliable test which would quantitate the CSF immunoglobulins. The standard for accuracy for all these procedures has been the immunochemical method of Kabat et al.2 Few clinical laboratories use this test, however, for it is time consuming, expensive, and complex. In 1966, a method of electroimmuno-diffusion (EID) suitable for use with minute volumes of unconcentrated CSF was described by Hartley et al.3 It is the purpose of this paper to relate our clinical experience with this test, to compare it with other methods for quantitation of CSF immunoglobulins, and to summarize the available information regarding the value of CSF

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