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October 1969

Necrosis and Regeneration of the Tibialis Anterior Muscle in Rabbit: II. Biochemical Changes: Lactate Dehydrogenase, Creatine Kinase, and Their Isoenzymes in Serum and Muscle

Author Affiliations

Berne, Switzerland
From the departments of pediatrics (Dr. Wiesmann) and neurology (Drs. Kaspar and Mumenthaler), University of Berne, Berne, Switzerland.

Arch Neurol. 1969;21(4):373-380. doi:10.1001/archneur.1969.00480160045005

LIGATION of the anterior tibial artery in rabbits results in ischemic necrosis of the distal two thirds of the anterior tibial muscle. Regeneration of the muscle fibers is almost complete within 90 days. The process of morphological regeneration is described in detail in previous papers.1,2 Biochemical studies during necrosis and regeneration of the muscle fibers are presented in this paper.

Materials and Methods  Fifteen adult rabbits of different species, origin, and sex, weighing between 2,350 gm (5 Ib 3 ounces) and 5,100 gm (11 Ib- 4 ounces) were used. The anterior tibial artery of the right hind limb was ligated according to the method of Clark et al.3 The animals were allowed to survive 1, 4, 6, 8, 10, 25, 35, 45, and 90 days and then were killed. Both anterior tibial muscles were removed immediately after death and treated as previously described.2 The muscles of

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