An electroimmunodiffusion method has been standardized for the simultaneous determination of IgG and albumin in cerebrospinal fluid (CSF) and serum. It is reliable and as valid as the most precise available method, Kabat's immunochemical technique. The procedure requires only 5μl of normal CSF (unconcentrated) or normal serum diluted 200 times. The lgG/albumin quotient is as discriminative for multiple sclerosis as the accepted ratio, lgG/total protein. The proposed procedure to estimate the CSF lgG/albumin quotient is suitable for any clinical laboratory that processes CSF.
Wallace W. Tourtellotte, Bruno Tavolato, Julius A. Parker, Patria Comiso. Cerebrospinal Fluid ElectroimmunodiffusionAn Easy, Rapid, Sensitive, Reliable, and Valid Method for the Simultaneous Determination of Immunoglobulin-G and Albumin. Arch Neurol. 1971;25(4):345–350. doi:10.1001/archneur.1971.00490040071008