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July 1979

Gomori's Trichrome Stain

Author Affiliations

Department of Neurology Baylor College of Medicine Houston, Texas

Arch Neurol. 1979;36(7):454-455. doi:10.1001/archneur.1979.00500430084024

To the Editor.—  We read with interest the article by Dr M. L. Grunnet (Archives 35:692, 1978) on Gomori's trichrome stain and its use with myelin sheaths. The fact that the myelin sheath of intramuscular nerves stains red with modified Gomori's trichrome stain is well known to muscle pathologists.1 Like Dr Grunnet, we have used this stain as a useful tool in the evaluation of more than 100 peripheral nerve biopsy samples in the past three years. However, to avoid an unacceptable amount of distortion and shrinkage of nerve fascicles caused by formaldehyde fixation,2 we, unlike the author, prefer to apply this stain to longitudinal and transverse frozen sections of a portion of nerve specimen. With this technique, one also eliminates the trouble of paraffining and deparaffining nerve samples and can easily identify endoneurium, perineurium, epineurium, vasa nervorum, and gross density of myelinated fibers (Figure, left). At higher

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