We appreciate Dr Keshgegian's comments. Following the publication of our report on immunologic abnormalities in the CSF of patients with progressive myoclonus epilepsy, we developed a micromethod for detection of oligoclonal IgG in unconcentrated CSF by polyacrylamide gel electrophoresis.1 We tested for oligoclonal IgG bands in the same samples (a small amount of CSF was available from each of the 16 patients with progressive myoclonus epilepsy) using our micromethod. A positive finding was detected in 12 of 16 patients. The indirect immunofixation technique, including surface fixation with specific antiserum2 and peroxidase-tagged antiglobulin with diaminobenzidine,3 demonstrated that these bands were immunoglobulins of the γ-G class.
Iivanainen M, Leinikki PO, Taskinen EI, Shekarchi IC, Madden DL, Sever JL. Oligoclonal Bands in Myoclonus Epilepsy-Reply. Arch Neurol. 1982;39(5):321. doi:10.1001/archneur.1982.00510170063024
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