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August 1987

Anti-Purkinje Cell Antibody Producing B-Cell Lines From a Patient With Paraneoplastic Cerebellar Degeneration

Author Affiliations

From the Departments of Neurology (Dr Tsukamoto), Bacteriology (Dr Yoshie and Mr Tada), and Neuropathology (Dr Iwasaki), Tohoku University School of Medicine, Sendai, Japan.

Arch Neurol. 1987;44(8):833-837. doi:10.1001/archneur.1987.00520200037015

• Two patients with paraneoplastic cerebellar degeneration with anti-Purkinje cell antibodies are described. Using an avidin-biotin peroxidase complex method, serum anti-Purkinje cell antibody titers of these patients were 1:32 000 and 1:64000, and the cerebrospinal fluid titers were 1:40 and 1:320, respectively. After extensive plasmapheresis together with radiation therapy and chemotherapy, the cerebellar symptoms of one patient ameliorated concomitantly with a reduced serum anti-Purkinje cell antibody titer. Four B-cell lines producing anti-Purkinje cell antibodies were established from one patient by Epstein-Barr virus trasformation of peripheral lymphocytes. None of these antibodies reacted with peripheral T cells, although the serum anti-Purkinje cell activity of the patient was markedly reduced after absorption with purified human T cells. Thus, anti-Purkinje cell antibodies are apparently directed both to antigens unique to the cerebellar tissue and those shared by T cells.