• We have established an interleukin 2-dependent, OKT4-positive T-cell line, named HCT-1, from cells in the cerebrospinal fluid obtained from a patient with human T-cell lymphotropic virus type I (HTLV-1)-associated myelopathy. Antigens for HTLV-I were detected in HCT-1 cells by indirect immunofluorescence and Western blot testing, and type C virus particles were detected by electron microscopy. Southern blot analysis of HCT-1 cellular DNA, using an HTLV-I probe, revealed that the integrated provirus genome could not be distinguished from the HTLV-I genome in adult patients with T-cell leukemia.
Nakamura T, Tsujihata M, Shirabe S, Matsuo H, Ueki Y, Nagataki S. Characterization of HTLV-I in a T-Cell Line Established From a Patient With Myelopathy. Arch Neurol. 1989;46(1):35–37. doi:10.1001/archneur.1989.00520370037015
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