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This book discusses the neuroanatomic tracer technique that uses the uptake and transport of the lectin, Phaseolus vulgaris—leucoagglutinin (PHA-L). The authors, Drs Wouterlood and Groenewegen, have had extensive experience employing the methods discussed in the book as well as providing a number of significant contributions to the field of neuroanatomic research. Overall, this book is a well-written and state-of-the-art description of the use of the PHA-L tracer techniques in neuroscience today. It is applicable for those new to tracer methods as well as for veterans in the field.
In the introduction, the authors list the characteristics of the ideal neuroanatomic tracer. They then proceed to examine each feature with respect to PHA-L tracing. The authors discuss several critical aspects of PHA-L tracer methodology, such as injection parameters and mechanisms of uptake, tissue preparation, visualization of transported PHA-L, rate of anterograde transport, retrograde transport, and uptake by fibers-of-passage. Included also
Romagnano MA. The Phaseolus vulgaris—Leucoagglutinin Tracing Technique for the Study of Neuronal Connections. Arch Neurol. 1992;49(10):1005. doi:10.1001/archneur.1992.00530340019006
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