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This book is an excellent companion to the first book published by these editors, In Situ Hybridization: Applications to Neurobiology. The current text describes the recent methodologic challenges facing researchers who use in situ hybridization (ISH). After a brief description of the technique, subsequent chapters describe advances in more complicated applications of ISH, such as combined ISH and receptor autoradiography, dual labeling of messenger RNAs (mRNAs) and/or proteins using both isotopic and nonisotopic methods, and simultaneous analysis of peptide secretion and gene expression. This is followed by two chapters that deal with the important and timely issue of quantification of mRNAs using ISH. In addition, there are chapters that discuss the measurement of nuclear mRNAs (heteronuclear RNA) and the use of ISH in identifying alternatively transcribed mRNAs in Alzheimer's disease. In an attempt to address the issue of sensitivity when using ISH, there is a chapter devoted to the use