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Comment & Response
October 2013

Detection of Antiretinal Autoantibodies in Serum by Western Blotting–Reply

Author Affiliations
  • 1Massachusetts Eye and Ear Infirmary, Boston
JAMA Ophthalmol. 2013;131(10):1372. doi:10.1001/jamaophthalmol.2013.5369

In Reply We thank Drs Adamus and Wilson for their comments on our article. They state that because testing method details were not provided, the provided results cannot be truly compared. The word limit for our article did indeed limit our ability to describe in detail the 2 laboratories’ methods. However, it is still valid to compare the results. If the purpose of our study was to compare the results between 2 laboratories performing the same method, then this would not have been a valid comparison. However, we know that the methods for ARA testing vary from laboratory to laboratory. The purpose of our study was to determine whether these different methods translate into different results to the ordering ophthalmologist in the real-world clinical setting. We agree with Drs Adamus and Wilson that our result was not particularly surprising, but we thought it was important to perform such a comparison to raise awareness among practicing clinicians so that they might evaluate the methods and results of their laboratory critically.