Tear Production After Unilateral Removal of the Main Lacrimal Gland in Squirrel Monkeys | Cornea | JAMA Ophthalmology | JAMA Network
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Farris  RL Abnormalities of the tears and treatment of dry eyes. Kaufman  HEBarron  BAMcDonald  MBeds. The Cornea. 2nd ed. Boston, Mass Butterworth-Heinemann1998;109- 129Google Scholar
Lemp  MAWolfley  DE The lacrimal apparatus. Hart  WM  Jred. Adler's Physiology of the Eye. 9th ed. St Louis, Mo Mosby–Year Book Inc1992;18- 28Google Scholar
Jones  LT The lacrimal tear system and its treatment.  Am J Ophthalmol. 1966;6247- 60Google Scholar
Jordan  ABaum  J Basic tear flow: does it exist?  Ophthalmology. 1980;87920- 930Google ScholarCrossref
Lemp  MA Recent developments in dry eye management.  Ophthalmology. 1987;941299- 1304Google ScholarCrossref
Stein  RHurwitz  JJ Anatomy and physiology of tear secretion. Hurwitz  JJed. The Lacrimal System. New York, NY Lippincott-Raven1995;1- 8Google Scholar
Beuerman  RWMcDonald  MBZhang  DVarnell  RJThompson  HW Diclofenac sodium attenuates neural activity after photorefractive keratectomy in rabbits.  J Refract Surg. 1996;12783- 791Google Scholar
Beuerman  RWMaitchouk  DYVarnell  RJPedroza-Schmidt  L Interactions between lacrimal function and the ocular surface. Kinoshita  SOhashi  Yeds. Proceedings of the 2nd Annual Meeting of the Kyoto Cornea Club. The Hague, the Netherlands Kugler Publications1998;1- 10
Mathers  WDLane  JASutphin  JEZimmerman  MB Model for ocular tear film function.  Cornea. 1996;15110- 119Google ScholarCrossref
Arffa  RC Diseases of the Cornea.  St Louis, Mo Mosby–Year Book Inc1991;
van Haeringen  NJ Clinical biochemistry of tears.  Surv Ophthalmol. 1981;2684- 96Google ScholarCrossref
Sullivan  DASato  EH Potential therapeutic approach for the hormonal treatment of lacrimal gland dysfunction in Sjogren's syndrome.  Clin Immunol Immunopathol. 1992;649- 16Google ScholarCrossref
Holly  FJLemp  MA Tear physiology and dry eyes.  Surv Ophthalmol. 1977;2269- 87Google ScholarCrossref
van Bijsterveld  OP Diagnosis and differential diagnosis of keratoconjunctivitis sicca associated with tear gland degeneration.  Clin Exp Rheumatol. 1990;8(suppl 5)3- 6Google Scholar
Mathers  WDLane  JAZimmerman  MB Tear film changes associated with normal aging.  Cornea. 1996;15229- 234Google ScholarCrossref
Danjo  YLee  MHorimoto  KHamano  T Ocular surface damage and tear lactoferrin in dry eye syndrome.  Acta Ophthalmol (Copenh). 1994;72433- 437Google ScholarCrossref
Vangveeravong  SKatz  SERootman  JWhite  V Tumors arising in the palpebral lobe of the lacrimal gland.  Ophthalmology. 1996;1031606- 1612Google ScholarCrossref
Mombaerts  ISchlingemann  ROGoldschmeding  RNoorduyn  LAKoornneef  L The surgical management of lacrimal gland pseudotumors.  Ophthalmology. 1996;1031619- 1627Google ScholarCrossref
Massry  GGHarrison  WHornblass  A Clinical and computed tomographic characteristics of amyloid tumor of the lacrimal gland.  Ophthalmology. 1996;1031233- 1236Google ScholarCrossref
Takeshita  SSugai  SOgawa  Y  et al.  A case of Sjogren's syndrome with an eyelid tumor, a so-termed pseudolymphoma of the lacrimal gland.  Ryumachi. 1996;3643- 49Google Scholar
Jakobiec  FAFont  RL Orbit: noninfectious orbital inflammation. Spencer  WHed. Ophthalmic Pathology: An Atlas and Textbook. Vol 3.3rd ed. Philadelphia, Pa WB Saunders Co1985;2777- 2795Google Scholar
Scherz  WDohlman  CH Is the lacrimal gland dispensable? keratoconjunctivitis sicca after lacrimal gland removal.  Arch Ophthalmol. 1975;93281- 283Google ScholarCrossref
Parsons  JTBova  FJMendenhall  WMMillion  RRFitzgerald  CR Response of the normal eye to high dose radiotherapy.  Oncology. 1996;10837- 847Google Scholar
Whitcher  JP Clinical diagnosis of the dry eye.  Int Ophthalmol Clin. 1987;277- 24Google ScholarCrossref
Mackie  IASeal  DV The questionably dry eye.  Br J Ophthalmol. 1981;652- 9Google ScholarCrossref
van Bijsterveld  OP Diagnostic tests in the sicca syndrome.  Arch Ophthalmol. 1969;8210- 14Google ScholarCrossref
Varnell  RJFreeman  JYMaitchouk  DBeuerman  RWGebhardt  BM Detection of substance P in human tears by laser desorption mass spectrometry and immunoassay.  Curr Eye Res. 1997;16960- 963Google ScholarCrossref
Maitchouk  DYVarnell  RJBeuerman  RWYan  J Analyzing proteins in tears from patients with dry eye syndrome, with contact lenses, or undergoing photorefractive keratectomy [abstract].  Invest Ophthalmol Vis Sci. 1996;37(suppl)S18Google Scholar
Beuerman  RWPedroza  L Ultrastructure of the human cornea.  Microsc Res Tech. 1996;33320- 335Google ScholarCrossref
Helper  LCMagrane  WGKoehm  JJohnson  R Surgical induction of keratoconjunctivitis sicca in the dog.  J Am Vet Med Assoc. 1974;165172- 174Google Scholar
McLaughlin  SABrightman 2nd  AHHelper  LCPrimm  NDBrown  MGGreeley  S Effect of removal of lacrimal and third eyelid glands on Schirmer tear test results in cats.  J Am Vet Med Assoc. 1988;193820- 822Google Scholar
Jester  JVNicholaides  NKiss-Palvolgyi  ISmith  RE Meibomian gland dysfunction, II: the role of keratinization in a rabbit model of MGD.  Invest Ophthalmol Vis Sci. 1989;30936- 945Google Scholar
Maudgal  PC The epithelial response in keratitis sicca and keratitis herpetica (an experimental and clinical study).  Doc Ophthalmol. 1978;45223- 327Google ScholarCrossref
Stephens  LCSchultheiss  THEAng  KKGray  KNPeters  LJ Anatomy of the major lacrimal gland of rhesus monkeys (Macaca mulatta).  J Med Primatol. 1987;16407- 419Google Scholar
Gilbard  JPRossi  SRGray  KL A new rabbit model for keratoconjunctivitis sicca.  Invest Ophthalmol Vis Sci. 1987;28225- 228Google Scholar
Mackor  AJvan Bijsterveld  OP Tear function parameters in keratoconjunctivitis sicca with and without the association of Sjogren's syndrome.  Ophthalmologica. 1988;196169- 174Google ScholarCrossref
Yoshino  KMonroy  DPflugfelder  SC Cholinergic stimulation of lactoferrin and epidermal growth factor secretion by the human lacrimal gland.  Cornea. 1996;15617- 621Google ScholarCrossref
Nakamori  KOdawara  MNakajima  TMizutani  TTsubota  K Blinking is controlled primarily by ocular surface conditions.  Am J Ophthalmol. 1997;12424- 30Google Scholar
McGill  JILiakos  GMGoulding  NSeal  DV Normal tear protein profiles and age-related changes.  Br J Ophthalmol. 1984;68316- 320Google ScholarCrossref
Newell  FW Ophthalmology.  St Louis, Mo CV Mosby Co1986;42- 80
Vanley  GTLeopold  IHGregg  TH Interpretation of tear film breakup.  Arch Ophthalmol. 1977;95445- 448Google ScholarCrossref
Seifert  PSpitznas  M Demonstration of nerve fibers in human accessory lacrimal glands.  Graefes Arch Clin Exp Ophthalmol. 1994;232107- 114Google ScholarCrossref
Seifert  PSpitznas  MKoch  FCusumano  A Light and electron microscopic morphology of accessory lacrimal glands.  Adv Exp Med Biol. 1994;35019- 23Google Scholar
Yoshino  KTseng  SCPflugfelder  SC Substrate modulation of morphology, growth, and tear protein production by cultured human lacrimal gland epithelial cells.  Exp Cell Res. 1995;220138- 151Google ScholarCrossref
Karas  MBahr  UGiebmann  U Matrix-assisted laser desorption ionization mass spectrometry.  Mass Spectrom Rev. 1992;10335- 357Google ScholarCrossref
Van Setten  GBSchultz  GSMacauley  S Growth factors in human tear fluid and in lacrimal glands.  Adv Exp Med Biol. 1994;350315- 319Google Scholar
Van Setten  GSchultz  G Transforming growth factor–alpha is a constant component of human tear fluid.  Graefes Arch Clin Exp Ophthalmol. 1994;232523- 526Google ScholarCrossref
Laboratory Sciences
February 2000

Tear Production After Unilateral Removal of the Main Lacrimal Gland in Squirrel Monkeys

Author Affiliations

From the Department of Ophthalmology, Lions Eye Research Laboratories, Laboratory for the Molecular Biology of the Ocular Surface, LSU Eye Center, Louisiana State University Health Sciences Center, New Orleans (Drs Maitchouk, Beuerman, Ohta, and Varnell); and Allergan Pharmaceuticals, Irvine, Calif (Dr Stern). None of the authors has any financial or proprietary interests in any of the products or instruments used in this study.

Arch Ophthalmol. 2000;118(2):246-252. doi:10.1001/archopht.118.2.246

Objective  To study the effects of lacrimal gland removal on basal and reflex tear production and on the ocular surface in the squirrel monkey.

Methods  Unilateral main lacrimal gland removal in 6 squirrel monkeys was followed by Schirmer testing, slitlamp examination with fluorescein, and collection of basal and reflex (stimulated) tears for analysis of tear protein spectra between 0 and 20 kd, as well as histological evaluation.

Results  Schirmer test results showed an 80% decrease in basal tears and a 90% decrease in reflex tears during week 1, and a 32.2% and 33.3% decrease, respectively, at week 20 after surgery, compared with the contralateral control side. However, no gross abnormalities or fluorescein staining were seen in 5 of the 6 monkeys, and the conjunctival surfaces remained normal. The main and accessory lacrimal glands appeared to secrete similar types of proteins. No histological changes were seen in corneal, conjunctival, or eyelid tissues 20 weeks after surgery.

Conclusions  Tears from accessory lacrimal glands were sufficient to maintain a stable tear layer on the cornea, suggesting that so-called basal tear flow is made up of fluid from both main and accessory lacrimal glands and that decreased tear production by the main lacrimal gland is not a causative factor in keratoconjunctivitis sicca.

Clinical Relevance  This study shows that total removal of the main lacrimal gland does not in itself lead to keratoconjunctivitis sicca. However, the nature of neural control of the accessory glands is not yet clear.