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Article
December 1931

ISO-ELECTRIC POINT OF LENS PROTEIN

Author Affiliations

IOWA CITY
From the Department of Ophthalmology, College of Medicine, State University of Iowa.

Arch Ophthalmol. 1931;6(6):870-876. doi:10.1001/archopht.1931.00820070901005
Abstract

From the report of Scalinci,1 it appears that Gullotta was the first to make an attempt to determine the iso-electric point of lens protein. Gullotta's method in brief was as follows : Solutions of different hydrogen ion concentrations were prepared with acetic acid and sodium acetate. Ox lenses were kept in these solutions for a certain period of time, and the increase in weight was ascertained at the end of the experiment. The minimum absorption of water was found to occur at pH 4, and this was regarded as the iso-electric point of the lens protein. Scalinci, using Gullotta's method, made a separate determination of the iso-electric point of the cortex and nucleus of the lens. He reported the following pH values: cortex, from 3.5 to 4; nucleus, 4.5. This gives an average pH value of 4 for the whole lens protein and agrees with

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