Numerous methods have been described for the preparation of pathologic material for microscopic study, but all are not efficacious for ocular material because of the difference in density of the ocular tissues. Frequent experimentation has revealed varied results, with the usual return to the common method of embedding in celloidin.
I have tried every process suggested to me, including the dry method with the block prepared in cedar oil, the slow method which requires from four to six weeks for the infiltration of the celloidin, the hot celloidin method and many others, all of which present disadvantages.
The most satisfactory method that I have employed is the one suggested by Prof. George de Renyi of the University of Pennsylvania. With modifications, I have found it the most practical because of the saving in time, the thinness of the sections and the manner of handling the block, which
De Long P. A NEW TECHNIC FOR THE PREPARATION OF PATHOLOGIC SPECIMENS FOR MICROSCOPIC STUDY. Arch Ophthalmol. 1933;9(6):976–978. doi:10.1001/archopht.1933.00830011000013
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