The growth of tissues or cells in vitro depends on the viability of the explant and on the conditions prevailing in the culture medium. Study of the metabolism of the tissue in vitro is aided by additions to or variations in the composition of the culture medium.
If the observed results of such a study are used as a corollary for the behavior of the tissue under like nutrient conditions when part of the living organism, certain qualifying characteristics of cells growing in vitro have to be taken into account: 1. The increase in size of a tissue cultured in vitro takes place mainly by proliferation with or without enlargement of the cells and by an increase in the intracellular material. 2. When the plasma clot method is used, the cells of a tissue culture are not provided with a constantly changing fluid nutrient medium as are the cells in
KIRBY DB, ESTEY KC, WIENER RVE. EFFECT OF CHANGES IN MEDIUM ON CULTURES OF LENS EPITHELIUM: STUDY IV. Arch Ophthalmol. 1933;10(1):37–41. doi:10.1001/archopht.1933.00830020045007
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