This paper is a preliminary report of an experimental study of some reactions of isolated iris muscles, a new autographic method being used. Both physiologic and pharmacologic stimulators and depressors were employed. The delicate method showed physiologic tissue response and permitted pharmacologic instead of toxic reactions.
METHOD AND APPARATUS
The sections of iris were taken from the enucleated eyes of anesthetized or of stunned animals, mounted with clips and at once suspended in oxygenated Ringer's solution. The temperature was controlled (from 32.7 to 33.2 C.) and also the ph (from 7.5 to 7.7, LaMotte colorimetric method). The suspension was fixed below and was attached above by a delicate thread to a galvanometer at a controllable tension. To increase the load of work, the muscles contracted against the elasticity of delicate coiled springs, and conversely a return to a relaxed state was favored by the stored energy in the springs.