In histologic sections of the eye it is not possible to identify certain cell components by ordinary microscopic study in visible light. Some of the substances which cannot be seen are of decisive importance for the physiologic function of the eye—for example, vitamin A. Many other cell components and tissue structures can be delineated only after the use of various complicated staining methods which require considerable time. In filtered ultraviolet radiation various substances that have a similar appearance in white light can be readily differentiated owing to their characteristic fluorescence. By utilizing this phenomenon in microscopic examination it is possible to identify a great number of cell components ; also it is possible by simple fluorescent staining to differentiate between a variety of cell structures and tissue elements, thereby greatly facilitating the explanation of the function of tissues on the basis of their micromorphologic appearance. This type of microscopic study has
EVANS JN, SINGER E. FLUORESCENCE MICROSCOPY APPLIED TO OCULAR TISSUES. Arch Ophthalmol. 1941;25(6):1007–1019. doi:10.1001/archopht.1941.00870120089005
Customize your JAMA Network experience by selecting one or more topics from the list below.