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February 1947


Arch Ophthalmol. 1947;37(2):182-188. doi:10.1001/archopht.1947.00890220191009

STUDIES of the blood vessels of the human retina were primarily stimulated by interest in the anatomy of the structures of the eyeground as they relate to basic aspects of ophthalmoscopy. The paucity of information to be gathered from the literature on the histology of the retinal vascular system made it necessary to undertake investigations of surface preparations of the choroid and the retina.

A number of these preparations were stained with a great variety of agents before an occasional satisfactory one was obtained. Most of the material was obtained from eyes enucleated for the usual clinical indications. The retinal preparations found most useful were those stained with Weigert's stain for elastic tissue without counterstain. Fixation was with dilute solution of formaldehyde U. S. P. The best preparations of the choroid were those stained with Mallory's aniline blue. The retina or the choroid was stripped from the underlying layer. In

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