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August 1949

MECHANISM OF CORNEAL WOUND HEALING: I. Cells Involved in Corneal Growth and Repair

Author Affiliations

From the Department of Surgery of the New York Hospital-Cornell Medical Center.

Arch Ophthalmol. 1949;42(2):140-147. doi:10.1001/archopht.1949.00900050145004

IN SPITE of the large amount of clinical and laboratory research work1 that has been done, very little is actually known about the mechanism of corneal wound healing and the cells involved in this healing process. To this end we have aimed our research: first, to identify the cells involved and, second, to learn the mechanism of corneal wound healing.

METHOD  A method has been worked out for maintaining rabbit corneal tissue in vitro apparently for an indefinite period. Fresh rabbit cornea is removed from the eye of the rabbit. The cornea is cut into pieces approximately 2 mm. square and 0.5 mm. thick. These pieces of tissue are planted in 3.5 D. Carrel flasks containing as a basic medium 0.7 cc. of Tyrode's solution, 0.3 cc. of rabbit serum, 0.5 cc. of chicken plasma and 1 drop of tissue extract of chick embryonic tissue (to facilitate formation of

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