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April 1950

EXPERIMENTAL STUDY ON THE VITREOUS: II. Experiments on Disappearance of Red Blood Cells from the Vitreous

Author Affiliations

From the Department of Ophthalmology of Columbia University College of Physicians and Surgeons, and the Institute of Ophthalmology of the Presbyterian Hospital in the City of New York.

Arch Ophthalmol. 1950;43(4):638-652. doi:10.1001/archopht.1950.00910010649003

EFFECT OF HYALURONIDASE  THE PREVIOUSLY reported experiments on isolated pig and steer vitreous1 indicated that the decrease of viscosity due to autolytic processes enhanced diffusion of homologous hemoglobin in this substrate. No liquefying or depolymerizing effect of the enzyme hyaluronidase on hyaluronic acid—the viscous component of the vitreous jelly—could be demonstrated by the spreading rate of hemoglobin in fresh steer vitreous. However, these results could not be applied directly to conditions in the living eye of another species. In the first part of the present study the liquefying effect of hyaluronidase was examined on the vitreous of rabbit eyes in vivo. In the main experimental series the effect of the mycolytic enzyme on the disappearance of rat cells from the vitreous was investigated.

TECHNIC  Washed red blood cells served as the test substance in rabbits. A sample of the animal's own blood was withdrawn from the heart or from

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