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June 1954

CAPILLARY TUBE METHOD FOR ANTIBODY DETERMINATION: Preliminary Studies in Aqueous of Rabbits Sensitized with Various Antigens

Author Affiliations

From the Department of Ophthalmology, the Ohio State University College of Medicine.

AMA Arch Ophthalmol. 1954;51(6):875-877. doi:10.1001/archopht.1954.00920040885013

THE SMALL amount of aqueous humor which is available for titration of antibodies is probably the chief factor that precludes the study of the ocular immunologic manifestations in sensitized animals. This investigation was an attempt, therefore, to apply an immunologic technique whereby the aqueous humor of one eye would be sufficient for antibody titration. Since the adaptability of the capillary tube method to immunologic studies has been demonstrated by Chown1 for detecting the presence of Rh antibodies and by King2 for conducting the milk test for brucellosis, this method was employed for determination of antibodies in the aqueous humor.

METHODS AND MATERIALS  Albino rabbits of varying weights and of both sexes were used throughout the study. Four animals were sensitized to bovine albumin * by three intravenous and by three intramuscular injections of 0.2 ml. of the antigen, at four-day intervals. Four other animals were sensitized in the same

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