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March 1955

Paraffin Method of Embedding Ocular Specimens for Microscopic Study

Author Affiliations

New York
From the Laboratories and Ophthalmological Division of the Mount Sinai Hospital.

AMA Arch Ophthalmol. 1955;53(3):365-368. doi:10.1001/archopht.1955.00930010367006

Paraffin as a medium for embedding ocular tissues is used to a considerable extent in many eye laboratories. While formerly used only for biopsy and autopsy specimens, it is now employed by some for surgical specimens as well. At the Mount Sinai Hospital in New York, we have used paraffin for many years for surgical specimens. Even large, bulky specimens of exenterated tissue are treated by this method. The method has proved to be so satisfactory that in recent years it has replaced celloidin (or Parlodion) embedding as the method of choice in the preparation of all ocular specimens.

Whole sections of surgically removed eye specimens prepared in celloidin have undoubted advantages in that the shape of the globe undergoes the least possible alteration and the relative positions of ocular layers are undisturbed, probably as a result of the slow embedding process. Probably the only additional advantage of celloidin is

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