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April 1955

Determination of Acetazoleamide in Aqueous Humor of the Rabbit Eye

AMA Arch Ophthalmol. 1955;53(4):478-480. doi:10.1001/archopht.1955.00930010480003

In two previous papers * it had been shown that the intravenous and subconjunctival injection of aceatzoleamide (Diamox) completely inhibited the carbonic anhydrase activity of the anterior uvea of the rabbit eye, without appreciably lowering either the normal intraocular tension or the concentration of bicarbonate ion in the aqueous humor.

A better understanding of the mechanism of action of acetazoleamide in the management of glaucoma depends upon a knowledge of the medium through which the drug functions. It was of interest, therefore, to determine whether the drug entered the aqueous humor of the eye after local and systemic administration, and in what concentration.

The only method available for the determination of acetazoleamide in biological fluids was that of Maren, Ash, and Bailey.† It is based essentially upon the changing pH method of Philpot and Philpot4 and is dependent on the rate of hydration of CO2 in the presence of