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April 1955

Preservation of Cornea by Dehydration: A Preliminary Report

AMA Arch Ophthalmol. 1955;53(4):519-521. doi:10.1001/archopht.1955.00930010525009

The operation of keratoplasty is frequently limited by the unavailability of donor corneal material. This report concerns preliminary experiments on the storage of cornea by dehydration and its successful use in lamellar transplantation in animals.

Many attempts have been made to store corneal grafts over long periods, and various methods have been tried in animals, without success. Katzin (1947),1 Leopold and Adler (1947),2 and Smelser and Ozanics (1946)3 all used rapid freezing in liquid nitrogen, with and without isopentane as an intermediate heat-transfer medium. In the method described in the first two papers, the grafts, after freezing, were dried in vacuo over P2O5 at -40 C. They were reconstituted before use, in most cases, by the addition of isotonic sodium chloride solution. Smelser and Ozanics did not dry their materials but stored them at -195 C for periods of from one hour up to