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March 1956

The Facility of Aqueous Outflow: A Comparison of Tonography and Perfusion Measurements In Vivo and In Vitro

Author Affiliations

St. Louis
From the Department of Ophthalmology, Washington University School of Medicine and the Oscar Johnson Institute (Dr. Becker).

AMA Arch Ophthalmol. 1956;55(3):305-312. doi:10.1001/archopht.1956.00930030309001

The perfusion methods of Bárány1 and Grant2 provide a direct experimental approach to the measurement of aqueous outflow in the enucleated eye. When the method is applied to human eyes, opportunities are afforded for comparing in vitro values for facility of outflow with those calculated from tonographic tracings. Such comparison has demonstrated remarkably similar average values for the two populations of normal human eyes.2

The fortunate finding that tonography could be applied to rabbit eyes * permitted comparing the in vitro and in vivo methods in this experimental animal. Furthermore, this comparison could be made more precise by examining the same eye before and after enucleation, thus avoiding individual variation. In addition, the intermediary step before enucleation of perfusion in the living animal has been utilized. The data obtained by such cannulation in the living eye provided additional values for intraocular pressure (Po), as well as the