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July 1956

Studies on Aqueous Passages in the Eye of the Rabbit

Author Affiliations

From the Western Reserve University School of Medicine and the University Hospitals of Cleveland, Division of Surgery, Department of Ophthalmology.

AMA Arch Ophthalmol. 1956;56(1):10-15. doi:10.1001/archopht.1956.00930040012002

It is the purpose of this paper to report observations on aqueous veins and on other possible aqueous passages in normal and pathological rabbit eyes.

Method  Kleinert (1953) applied an ingenious technique for the demonstration of aqueous veins in human eyes by the injection into the anterior chamber of 0.1% fluorescein in isotonic saline. The stained aqueous humor is then observed in darkness by means of ultraviolet light, which makes it stand out selectively against an almost entirely dark background. This method was used in our experiments. For low magnifications (loupe), a fluorescent lamp, which yields ultraviolet light only, proved to be satisfactory. For higher magnifications a slit lamp was used with a dark violet gelatin filter placed in the beam.The studies were done on chinchilla rabbits and albino rabbits (3 kg. or more), which were first subjected to tonometry. Their heads and legs were immobilized, and 0.5% tetracaine