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March 1958

Human Amnion-Cell Tissue Culture: A Study of Herpes Simplex and Adenovirus

Author Affiliations

Bethesda, Md.
The Howe Laboratory of Ophthalmology of the Massachusetts Eye and Ear Infirmary and the Department of Medicine of the Massachusetts General Hospital, Boston.

AMA Arch Ophthalmol. 1958;59(3):342-349. doi:10.1001/archopht.1958.00940040048005

Introduction  In the study of viruses infecting the eye, a method of virus isolation has been sought which is simple, inexpensive, and yet permits rapid and reliable identification of the virus. Amnion tissue seems to afford such a means of virus identification.1,2 1. Amnion is an inexpensive source of cells—readily prepared and maintained with commercially available media. 2. A single amnion can yield many hundred roller tubes, which may be stored so that a single amnion can furnish a supply of roller tubes for two to three months.3-5 3. A wide spectrum of viruses can be grown in amnion: the herpes zoster-varicella group, poliomyelitis virus, most ECHO viruses, many of the coxsackie group, croup-associated virus, measles, and others.1,3-5 4. Many viruses, especially herpes simplex and the adenoviruses, produce characteristic cytopathogenic changes that are readily detected. These changes are more easily seen and develop earlier than would be

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