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May 1958

The Distribution of Lipids in the Bovine Lens and CorneaA Histochemical Study

AMA Arch Ophthalmol. 1958;59(5):676-682. doi:10.1001/archopht.1958.00940060060006

As a result of biochemical analyses of lipids in the bovine lens and cornea, Krause1 and, more recently, D'Asaro et al.2 have been able to show that phospholipids form a major constituent of the lipid fractions from the bovine lens and cornea. In the light of such biochemical information it is naturally of interest to determine the histochemical picture of lipid and phospholipid distribution in these two avascular structures. Apart from Wislocki's histochemical study on the anterior segment of the eye of the rhesus monkey,3 in which reference is made to the distribution of Sudan blackpositive material in the cornea and lens, no other attempt to localize lipid in these tissues on a cytological level seems to have been made. Furthermore, it has been recently established that an affinity for Sudan black may, on occasion, also characterize certain nonlipid substances4; additional tests are therefore required before