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May 1958

Dialysis of the Aqueous Against Plasma

Author Affiliations

New York
From the Department of Ophthalmology, New York University Post-Graduate Medical School.

AMA Arch Ophthalmol. 1958;59(5):703-705. doi:10.1001/archopht.1958.00940060087009

The demonstration that the electrochemical potential of a substance in the aqueous is greater than the requirements predicted by membrane equilibrium is evidence of an active transport mechanism for the substance. The main difficulty in applying this criterion is the uncertainty of the theoretical requirements. To overcome this difficulty Duke-Elder et al.1 utilized the technique of direct dialysis of the two fluids against each other. By dialysis, these investigators demonstrated a transfer of sodium from aqueous to plasma, supporting the view that an excess of sodium is present in the aqueous. The present investigation was undertaken as a further study of aqueous-to-plasma dialysis.

Method  Adult male albino rabbits weighing from 2.5 to 3.5 kg. were utilized, and each eye was used only once for aqueous puncture. They were allowed food (Wayne Rabbit Pellets) and water without restriction. Twenty-four hours before use they were transferred from the regular animal room

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