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July 1959

Trachoma Infection in a Human Volunteer Produced by Egg-Cultured Virus

Author Affiliations

Virology Medical Laboratory, The Hebrew University-Hadassah Medical School (Mr. Bernkopf and Mr. Nishmi), and Department of Ophthalmology, Hadassah Hospital (Mrs. Maythar and Mr. Feitelberg).

AMA Arch Ophthalmol. 1959;62(1):33-34. doi:10.1001/archopht.1959.04220010037004

Successful cultivation of trachoma virus has recently been announced by Tang et al.1 Their claim has been confirmed by Collier and Sowa,2 who reported the isolation of 10 strains of trachoma virus from West Africa and the successful inoculation of a human volunteer. Strains of virus have since been isolated also by American workers in Saudi Arabia.3 The present communication reports the isolation of a strain of virus in the Middle East and the clinical Picture produced in a blind volunteer.

Scrapings of the tarsal conjunctiva from seven trachoma patients, suspended in broth saline containing 1,000μg. of streptomycin per milliliter were inoculated in the yolk sac of groups of five 6-day-old chick embryos. One egg inoculated with material from Patient 1 was found dead 10 days later. A suspension of the yolk sac, bacteriologically sterile, was passed on in 6-day-old embryonated eggs, producing regularly death of the

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