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December 1959

Some General Principles of Electron Microscopy

Author Affiliations

Washington, D.C.
From the Ophthalmic Pathology Branch, Armed Forces Institute of Pathology.; Special trainee of the National Institute of Neurological Disease and Blindness, National Institutes of Health (Dr. Fine).

AMA Arch Ophthalmol. 1959;62(6):931-934. doi:10.1001/archopht.1959.04220060003001

Throughout this issue of the Archives the reader will find the results, mainly in the form of micrographs, of morphologic studies of ocular tissues obtained with the electron microscope. The techniques employed in electron microscopy differ considerably from those of light microscopy. For the proper appreciation of these results obtained with this instrument some knowledge of the methods used in specimen preparation is required. The purpose of this presentation is to outline some of the principles, materials, and techniques of electron microscopy.

The resolution of the average human eye (i.e., the closest two points or lines can be together and still be perceived as separate) is considered to fall in the range of 0.1-0.2 mm. (100μ-200μ). This inherent limitation of the eye is overcome by use of magnifying lenses which refract light to "spread" these two points apart, so that intermediate points or lines may be distinguished as separate. Glass

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