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April 1960

The Design and Performance of a Perfusion System for the Culture of the Lens: Part II

Author Affiliations

Iowa City
From the Departments of Physiology and Ophthalmology, College of Medicine, State University of Iowa.

AMA Arch Ophthalmol. 1960;63(4):607-624. doi:10.1001/archopht.1960.00950020609003

Introduction  A closer approach to physiological normalcy can be obtained by using an open system for the culture of the lens.* Certain criteria are necessary to fulfill the definition of an open system. They are (1) a source for a constant supply of nutrient medium; (2) a constant flow of nutrient medium past the lens, and (3) a sink for the collection of the medium. By this method the lens is continually exposed to a fluid of constant composition. The possibility exists therefore that a steady state condition can be obtained in regard to the transport and metabolic rate processes of the lens. With these criteria in mind, the best approach to the design of such an open system appeared to utilize the principle of perfusion.In order to study the behavior of the lens under simulated in vivo conditions in a perfusion system over a period of several days,

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