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April 1960

Initial Studies of the Use of an Open System for the Culture of the Rabbit Lens: Part IV

Author Affiliations

Iowa City
From the Departments of Physiology and Ophthalmology, College of Medicine, State University of Iowa.

AMA Arch Ophthalmol. 1960;63(4):643-659. doi:10.1001/archopht.1960.00950020645005

Introduction  In the previous papers the design and performance of a perfusion system and the synthetic medium to be used in such a system have been presented.* This paper will describe initial experiments on the use of the system for the culture of the rabbit lens. Eight perfusion experiments were done, each one lasting from two to three days. The first three experiments were designed to establish an optimum flow rate and reduced glutathione (GSH) concentration which were then used for the last five perfusion experiments.

Materials and Methods  Male rabbits weighing 4.5 to 5.5 lb. of the New Zealand white albino strain were used. The animals were killed by air embolism and the eyes enucleated immediately. The method of removing the lens under sterile conditions has been described previously.†Stainless steel surgical loops were used to remove the lens from the anterior face of the vitreous with a minimum