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May 1960

Effect of Hormones on S35-Labeled Sulfate Uptake in Guinea Pig Eye and Skin Connective Tissues

Author Affiliations

New York
From the Department of Ophthalmology, College of Physicians and Surgeons, Columbia University.

AMA Arch Ophthalmol. 1960;63(5):761-766. doi:10.1001/archopht.1960.00950020763003

Previous autoradiographic studies of the guinea pig eye (Larsen,1 1959) have shown that changes in the uptake of S35 occur when guinea pigs are treated with certain hormones. As autoradiography is primarily a qualitative method, the investigations reported in this paper were undertaken to determine quantitatively the variations achieved under similar conditions. Cornea and sclera were chosen as most suitable for examination; biopsies of the skin were used for comparison as representatives of connective tissues elsewhere in the body.

Material and Methods  Guinea pigs, both male and female, weighing from 190 to 390 gm. were used. Radioactive sulfate (with 0.04% Na2SO4 as a carrier) was injected intraperitoneally into the animals. Some guinea pigs (37) were given 2.0 mc. S35 per kilogram of body weight; others (64) received only 0.15 mc. S35 per animal because they were also used for other purposes. All of