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May 1960

Microelectrophoresis on Agar of Normal and Pathological Aqueous Humor

Author Affiliations

Ghent, Belgium
From the Ophthalmological Clinic, University of Ghent.; Director: Professor Dr. J. François.

AMA Arch Ophthalmol. 1960;63(5):836-849. doi:10.1001/archopht.1960.00950020838014

The examination of the aqueous humor by electrophoresis is not yet used as a routine procedure. In fact, it is difficult because of the small quantities of protein and the large quantities of salts present in the fluid of the anterior chamber.

In the normal aqueous humor, the concentration of the water-soluble proteins varies generally between 10 and 50 mg. %, i.e., it is 20 to 100 times smaller than the optimal concentration of serum proteins used for routine paper electrophoresis.

For satisfactory electrophoresis, the percentage of protein of the solution should be sufficiently high, hence, the need to concentrate the fluid from 50 to 100 times. This in turn greatly raises the salt content above the normal 1% (approximately) to a degree that may cause protein precipitation, which makes concentration of the aqueous humor by simple evaporation impossible.

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