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September 1960

Incorporation of Tritium-Labeled Thymidine by Rabbit Corneal Endothelium

Author Affiliations

New York
From the Department of Ophthalmology, Columbia University, College of Physicians and Surgeons.

Arch Ophthalmol. 1960;64(3):443-446. doi:10.1001/archopht.1960.01840010445019

Introduction  The problem of regeneration and replacement of corneal endothelial cells is still unsolved. It is generally held that there is no evidence of mitosis in the endothelium of adult animals,1-4 although mitoses have been observed in young cats,1 and recently von Sallmann5 has reported a mitotic index of 2/100,000 in "young adult rabbits." Binder and Binder described only "amitotic" cell division in uninjured rabbit endothelium but both "mitotic and amitotic cell division" in the endothelium 12 hours after injury with a blunt spatula.3 Following irrigation of the anterior chamber by α-chymotrypsin, von Sallmann observed greatly increased mitotic activity in some localized areas of Descemet's endothelium.Harding et al. have recently shown that the very weak β-radiation from tritiated thymidine can be detected, by means of autoradiography, in whole-mount preparations of lens epithelium.6 By this method, incorporation of tritiated thymidine by any cell in the