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October 1960

The Preparation of Sections and Museum Specimens of Eyes Using a Polyester Resin

Author Affiliations

Vancouver, B.C., Canada
Department of Ophthalmology, Vancouver General Hospital (Dr. Smith).; Department of Pathology, Faculty of Medicine, University of British Columbia (Dr. Culling).; Assistant Resident, Department of Ophthalmology, Vancouver General Hospital (Dr. Martin).

Arch Ophthalmol. 1960;64(4):558-565. doi:10.1001/archopht.1960.01840010560013

Examination of the eye histopathologically has long been recognized as a difficult procedure, due mainly to the fact that it is composed of several mixed hard and soft tissue layers which tend to separate during cutting. Furthermore, since a section of the entire globe is desirable this results in a large sized block which due to its hardness does not always permit even sections to be cut unless the embedding medium is an excellent one. An ideal embedding medium therefore is one that (a) does not crumble under the impact of knife on sclera and lens, (b) preserves the cytologic detail of the retina and other tissues, (c) permits a large block of tissue to be cut evenly and completely, (d) permits reasonably thin sections to be cut, and (e) gives a completed section in a reasonable time. While paraffin embedding will occasionally satisfy most of these criteria it usually