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April 1961

Tritium Retention by Corneal Endothelium After Incorporation of H3—Thymidine

Author Affiliations

New York
Departments of Ophthalmology and Physiology, College of Physicians and Surgeons, Columbia University.; Predoctoral fellow of the National Institute of Neurological Diseases and Blindness (Mr. Bito).

Arch Ophthalmol. 1961;65(4):553-556. doi:10.1001/archopht.1961.01840020555017

A program of study on DNA synthesis and mitosis in normal and injured tissues is being carried out in this laboratory. It has been demonstrated that injuries to the lens epithelium or corneal endothelium can stimulate the incorporation of tritium-labeled thymidine by many cells.1,2 The accumulated evidence from other studies has suggested that thymidine is a specific precursor to DNA synthesis and is incorporated into cells that are undergoing chromosome duplication in preparation for cell division.3,4 It is furthermore assumed that the tritium thus incorporated into cell nuclei is retained indefinitely by the cell5; a decrease in tritium per cell being accomplished, however, by subsequent cell divisions. Corneal endothelium apparently normally undergoes very little or no mitotic activity.6,10 For a limited period of time after mechanical injury, however, a number of cells in the vicinity of the wound show thymidine incorporation and division. After this period,

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