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April 1961

Distribution of Sialic Acids in the Human Eye

Author Affiliations

St. Louis
From the Department of Ophthalmology and the Oscar Johnson Institute, Washington University School of Medicine.; Special Fellow, National Institute of Neurological Diseases and Blindness, National Institutes of Health, U.S. Public Health Service (Dr. Haddad).

Arch Ophthalmol. 1961;65(4):563-564. doi:10.1001/archopht.1961.01840020565019

Histochemical studies suggest the presence of acid mucopolysaccharides in a number of ocular tissues.1-4 Sialic acids have been found in the aqueous and vitreous humors.5 In order to characterize the glycoproteins of other ocular tissues, analyses for sialic acids were made in different parts of the human eye.

Normal human eyes obtained at autopsy were provided by the eye bank. The anterior chamber was tapped first to collect the aqueous humor. The cornea was excised, and both epithelial and endothelial surfaces were scraped off. The lens was gently extracted, and its capsule was stripped off. The vitreous was collected in 2 portions: the more liquid anterior part and more viscous posterior fraction. The retina was stripped off the choroid and carefully cleared of adherent vitreous.

Each tissue was homogenized separately and heated with 0.1 N H2SO4 at 80 C for 1 hour. The sialic acids

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