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May 1961

In Vitro Procedure for Determining Transparency of Fresh and Preserved Donor Corneas

Author Affiliations

Durham, N.C.
Fight for Sight Research Fellow of the National Council to Combat Blindness, Inc., New York City (Dr. Matton-VanLeuven).; From the Duke University School of Medicine, Department of Surgery: Plastic Surgery Research Laboratory and Division of Ophthalmology.

Arch Ophthalmol. 1961;65(5):703-707. doi:10.1001/archopht.1961.01840020705018

The success of a full-thickness corneal graft with preserved donor tissue appears to depend mainly on 2 factors: (1) a viable and functioning endothelium which protects the stroma against fluid imbibition from the aqueous humor; (2) an intact stromal structure with normally distributed mucopolysaccharides.1,2 These 2 factors play an important role in the transparency of the donor corneas.

There have been few attempts to objectively determine corneal transparency. Usually the appearance of the donor cornea is subjectively evaluated by the surgeon.

Potts and Friedman3 devised a method for measuring the specular density of isolated beef corneas, which was practical at moderate corneal densities.

The present paper describes a method to evaluate corneal transparency over a wide range of densities. With this technique it was possible to compare the transparency of fresh corneas with that of frozen ones during their reconstitution after thawing and to study the influence of

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