Toxoplasma gondii is a parasitic protozoon which infects a wide range of natural hosts, including man. The infected hosts have been shown to produce specific antibodies which are usually detected by the methylene blue dye test and the complement fixation test.Another procedure based on hemagglutination methods outlined by Boyden (1951) and Stavitsky (1954) was reported by Jacobs and Lunde (1957), for detecting antibodies in toxoplasmosis using tanned sheep cells to which Toxoplasma antigen was adsorbed. As an antigen, they utilized an aqueous extract of toxoplasms which were obtained from the peritoneal fluid of infected mice. Later studies by Lunde and Jacobs (1959) have suggested that the antigen is a protein with thermolabile properties.The following modification of the above procedure has been developed in the Estelle Doheny Eye Foundation Laboratory. As tests to be performed were primarily with human serum samples, tanned human cells of Type O
LEWIS WP, KESSEL JF. Hemagglutination in the Diagnosis of Toxoplasmosis and Amebiasis. Arch Ophthalmol. 1961;66(4):471–476. doi:10.1001/archopht.1961.00960010473006
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