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January 1963

Comparison of Dye and Hemagglutination Tests on Sera of Suspected Cases of Toxoplasmic Uveitis

Author Affiliations

Bethesda, Md.; Baltimore
From the United States Department of Health, Education, and Welfare, Public Health Service, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Laboratory of Parasitic Diseases (Mr. Lunde and Dr. Jacobs) and Johns Hopkins University and Hospital, Wilmer Ophthalmological Institute (Dr. Wood).

Arch Ophthalmol. 1963;69(1):10-12. doi:10.1001/archopht.1963.00960040016004

Introduction  During the last several years, in a continuing collaborative study, we have performed dye and hemagglutination tests for toxoplasmosis on the sera of patients of the Wilmer Institute who presented signs of uveitis for which a diagnosis of toxoplasmosis was considered. The analysis here is presented as an evaluation of the usefulness of the hemagglutination test as a diagnostic means.The dye test was performed by the procedure used routinely in the Laboratory of Parasitic Diseases and described by Frenkel and Jacobs (1958).1 The hemagglutination test technique was essentially that given in our original description (Jacobs and Lunde, 1957) with the following exceptions: The saline solutions used were prepared differently (Lunde and Jacobs, 1958).3 Also, the HA antigen concentrations used were those that gave maximal titers with standard positive sera and at the same time gave unequivocally negative results with negative controls.The results are given in

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